基于微机械电子系统的电化学电极阵列的研制

为了克服传统电化学电极反应液需要量较大、预处理繁琐和耗时的不足,本研究通过腐蚀、键合、光刻、氧化、扩散和溅射等工艺在75 mm×25 mm光学玻片上制备了基于微机械电子系统的电化学电极阵列。该电极阵列含有16个电极单元,每个单元之间的差异性≤3.7%;电极表面Rct=28Ω,预处理时间1 min;反应液量1～50μL。用此电极阵列构建的生物传感器实现了对大肠杆菌特异性靶分子序列的检测,检测下限达到1×10-8mol/L。结果表明,此电化学电极阵列能满足多通道微量检测的需求,在其表面可方便地进行生物功能化修饰,应用范围广,实用性强。     

基于酶-抗体共固定二氧化锆纳米信号探针的瘦肉精安培免疫传感器研究

制备了基于酶-抗体共固定二氧化锆(ZrO2)纳米探针进行信号放大的盐酸特伦克罗瘦肉精(CLB)检测安培免疫传感器。首先,利用ZrO2纳米粒子负载葡萄糖氧化酶(Glucose oxidase,GOD)和CLB抗体(Clen-buterol antibody,anti-CLB),制得纳米探针(ZNPs/GOD-anti-CLB signal probe,简称ZNPG)。同时将聚二烯丙基二甲基氯化铵[Poly(diallyldimethylammonium chloride),PDDA]、氯化血红素(Hemin)和纳米金(AuNPs)层层组装到多壁碳纳米管(MWCNTs)修饰的丝网印刷电极(SPCE)表面,制得SPCE│MWCNTs/(PDDA/Hemin/AuNPs)n电流型传感器,可对H2O2产生还原催化电流。检测CLB时,将样品和ZNPG一起加入预先包埋好CLB的96孔板底部。样品CLB与板底CLB共同竞争结合孔溶液中的纳米探针ZNPG。反应结束后加入葡萄糖,板底ZNPG-CLB免疫复合物上的GOD催化葡萄糖氧化产生H2O2,可通过传感器检测。电流下降值(ΔI)与样品CLB成反比,可用于CLB的定量分析。由于ZNPG上具有高的GOD酶标记密度,故可以显著放大检测信号,提高检测灵敏度。在最佳条件下(pH 7.0,温育时间30 min,温育温度37℃),此传感器对CLB的检测范围为0.003～100μg/L,检测下限为1 ng/L,比酶联免疫分析法(ELISA)法灵敏度提高2个数量级,在猪饲料样品中进行加标回收实验,平均回收率达93.6%,相对标准偏差(RSD)小于2.5%,精密度好。同时该传感器具有可重复使用、灵敏快速,适合于食物样品中痕量CLB现场检测。     

4-羟基-5-乙氧基苯胺的合成

以苯胺为原料,经重氮化、偶合、还原得到4-羟基-5-乙氧基苯胺。合成中,通过保险粉还原偶氮化合物的方法在芳香环上引进氨基,不仅产物收率高(总收率86%,较文献提高了26.3%),而且还原产物容易分离,粗品纯度可达95%。本合成路线中间体均不经提纯,且氨基的引入中避免了贵金属的加氢还原,是一条适合大规模生产4-羟基-5-乙氧基苯胺的新合成工艺路线。     

Effect of hydrophobicity on the stability of sol–gel silica coatings in vacuum and their laser damage threshold

Silica antireflective coatings modified by hexamethyldisilazane (HMDS) were deposited on clean substrates (silicon wafer or K9 glass blanks) by sol–gel processing. The effects of HMDS on the contamination resistant capability and laser-induced damage threshold (LIDT) of coatings were investigated. Transmission electron microscopy revealed that a stable sol with uniformly distributed silica particles with an average particle size of about 15 nm was acquired by adding appropriate amount of HMDS into the standard SiO₂ sol. With the modified sol the resultant coatings were hydrophobic and the contact angle for water increased with increasing amount of HMDS in the reaction mixture. Such increase in hydrophobicity was not the result of surface roughness. The antireflective properties were retained after HMDS-treatment and the maximum transmission values were above 99 %. The introduction of HMDS into silica sols had also increased the LIDT of coatings from 24.3 to 37.0 J cm^?2 when the molar ratio of HMDS to tetraethoxysilane was 0.05:1. The increase in LIDT was attributed to the decrease of nodular defect and uniform microstructures of coatings as an effect of the HMDS modification. After some of the hydroxyl groups on the surface of the SiO₂ particle were replaced by methyl groups, from which the SiO₂ particle gained a water-repellent surface, the stability of coatings in vacuum was increased. The maximum transmission values of modified coatings decreased by only 0.25 % after storage under vacuum for 168 h. In contrast, the standard sol–gel silica coatings decreased about 2 % under similar conditions. The LIDT of modified coatings remained as high as 30.8 J cm^?2, more than that of standard coatings stored for the same duration in air.     

Stereoselective Synthesis of (+)-△~5-Dehydrosugiyl Methyl Ether

Most diterpenoids exhibit significant bioactivities1-4. (5-Dehydrosugiyl methyl ether was separated from Taxodium distichum Rich which showed significant bioactivity against KB5, and assigned the structure 1. This compound was obtained by Takashi MATSUMOTO from (+)-dehydroabietic acid with a known procedure6, but the synthetic route is too long. In order to study the relationship between the structure and bioactivities. The certain diterpene synthesis has been extended in our laboratory7…     

HPLC法测定食品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ

苏丹红是偶氮化合物类合成色素的一种。苏丹红Ⅰ化学分子式为C16H12ON2，苏丹红Ⅱ、Ⅲ、Ⅳ为苏丹红Ⅰ的化学衍生物，均有致癌性。2004年6月，欧盟批准了《有关辣椒及辣椒产品紧急措施的决定》，要求禁止进口掺有苏丹红的辣椒产品。     

比较和分析不同计算法中的人民币有效汇率

本文在用商品贸易权重计算有效汇率的基础上，探讨了用资本移动权重和债务权重计算有效汇率的新模型，并调查和实证分析了由这些模型决定的人民币有效汇率时序列运动以及它们与宏观经济变量之间的长期关系，以解决外汇管理中人民币升值压力上升问题。我们得出：在我国，基于贸易权重的实际有效汇率与基于债务权重的人民币实际有效汇率有较高的相关性，但基于贸易权重的与基于资本移动权重的没有相关性的结论。因此，我们认为在人民币升值压力较大时，货币当局可以用调整我国对外债务结构来替代词整对外贸易差额。     

二氰基二硫纶·5-硝基邻菲咯啉合钴(Ⅱ)的电子光谱及理论研究

报道了配合物Co(mnt)(5 NO2 phen) 在几种溶剂中的电子光谱,用RHF SCF MO理论的PM3方法进行了几何构型优化,研究了配合物基态和激发态的电子结构.由ZINDO/S方法的组态相互作用(CI)计算给出了理论光谱,计算结果与实测谱吻合较好.研究发现配合物紫外区的吸收带本质上属于配体的πb→π* 跃迁,可见光区400~500 nm存在本质上属于配体5 NO2 phen到配体mnt2-的荷移跃迁.     

基于XOR门的静态逻辑电路功耗优化技术

提出了一种新的基于XOR门的静态逻辑电路功耗优化技术.通过极性转换,可迅速得到基于XOR门的静态逻辑电路的最优结构,达到优化功耗的目的.实验结果表明,本文提出的算法在功耗节省方面比其他同类算法更有效.     

Numerical Analysis and Comparison of Three Metal-Oxide-Type Super-Resolution Near Field Structures

Based on the Fresnel-Kirchhoff diffraction theory, we build up a Gaussian diffraction model of metal-oxide-type super-resolution near field structure （super-liENS）, which can describe far field optical properties. The spectral contrast induced by refractive index and the structural changes in AgOg, PtOx and PdOz thin films, which are the key functional layers in super-RENS, are studied by using this model. Comparison results indicate that the spectral contrast depends intensively on the laser-induced distribution and change of the refractive index in the metal-oxide films. The readout mechanism of the metal-oxide-type super-RENS optical disc is further clarified. This Gaussian diffraction model can be used as a simple and effective method for choosing proper active materials in super-RENS.